Deleted
Sample Discontinued
Experience: None
Not Used
Get This Part
Plasmid_Backbone

Part:BBa_K209499

Designed by: Benjamin Rhau   Group: iGEM09_UCSF   (2009-10-19)

pEF5-FRT-V5-DEST

All ccdb parts have been withdrawn from the Registry. Samples of parts containing the ccdB gene cannot be requested. - iGEM HQ

Commercial plasmid used in the construction of other composite parts.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1628
    Illegal EcoRI site found at 2091
    Illegal EcoRI site found at 4289
    Illegal XbaI site found at 3380
    Illegal XbaI site found at 4021
    Illegal SpeI site found at 1611
    Illegal PstI site found at 665
    Illegal PstI site found at 1170
    Illegal PstI site found at 1633
    Illegal PstI site found at 3213
    Illegal PstI site found at 4379
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1628
    Illegal EcoRI site found at 2091
    Illegal EcoRI site found at 4289
    Illegal SpeI site found at 1611
    Illegal PstI site found at 665
    Illegal PstI site found at 1170
    Illegal PstI site found at 1633
    Illegal PstI site found at 3213
    Illegal PstI site found at 4379
    Illegal NotI site found at 1770
    Illegal NotI site found at 3367
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1628
    Illegal EcoRI site found at 2091
    Illegal EcoRI site found at 4289
    Illegal BglII site found at 12
    Illegal BglII site found at 919
    Illegal BglII site found at 2538
    Illegal BamHI site found at 1605
    Illegal BamHI site found at 1841
    Illegal BamHI site found at 2544
    Illegal XhoI site found at 1318
    Illegal XhoI site found at 3374
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1628
    Illegal EcoRI site found at 2091
    Illegal EcoRI site found at 4289
    Illegal XbaI site found at 3380
    Illegal XbaI site found at 4021
    Illegal SpeI site found at 1611
    Illegal PstI site found at 665
    Illegal PstI site found at 1170
    Illegal PstI site found at 1633
    Illegal PstI site found at 3213
    Illegal PstI site found at 4379
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1628
    Illegal EcoRI site found at 2091
    Illegal EcoRI site found at 4289
    Illegal XbaI site found at 3380
    Illegal XbaI site found at 4021
    Illegal SpeI site found at 1611
    Illegal PstI site found at 665
    Illegal PstI site found at 1170
    Illegal PstI site found at 1633
    Illegal PstI site found at 3213
    Illegal PstI site found at 4379
    Illegal NgoMIV site found at 1053
    Illegal NgoMIV site found at 3882
    Illegal NgoMIV site found at 5144
    Illegal AgeI site found at 431
    Illegal AgeI site found at 3447
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 3094
    Illegal BsaI site found at 3429
    Illegal BsaI.rc site found at 1567
    Illegal BsaI.rc site found at 6672
    Illegal SapI site found at 5589


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